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KMID : 0545120000100050620
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 5 p.620 ~ p.627
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Analysis of Genetic Polymorphisms of Epstein-Barr Virus Isolates from Cancer Patients and Healthy Carriers
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Cho Sung-Gyu
Lee Won-Keun
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Abstract
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To determine the prevalence of genetic polymorphisms in Epstein-Barr virus (EBV) strains in the Korean population, the restriction site polymorphisms for BamHI and XhoI enzymes were analyzed with 16EBV isolates from cancer patients and 7EBV isolates from healthy carriers, using polymerase chain reaction techniques. None of the 23 isolates were found to carry an extra BamHI site in the BamHI Ffragment (f-variant). Of the 12 type-1 isolates from the cancer patients, 10 lost both the LMP1 Viol site and the BamHI site between the BaniHI WI^* and I1^* fragments (a W1^*I1^* fusion variant or type C). The latter W1^*I1^* fusion variant was due to a mutation of thymidine to adenine, as evidenced by a sequence analysis. The remaining two type-1 isolates showed either no variation at both sites or the loss of only the XhoI site. In contrast, two type-2 isolates and two intertypic recombinants with a type-1 allele at the EBNA2 locus and type-2 alleles at all or some of the EBNA3 loci retained both enzyme sites. In similar analyses of the 7 isolates from the healthy carriers, five of six type-1 isolates lost these two sites, however, one type-2 isolate did not. These results clearly indicate a strong association of both the LMP1 XhoI site loss and the W1^*I1^* fusion variant with the type-1 rather than the type-2 EBV strains circulating in the immunocompetent Korean carriers.
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